HPLC法测定复方磺胺甲噁唑颗粒剂的含量

ASSAY OF COMPOUND SULFAMETHOXAZOLE GRANULES BY HPLC

目的:采用HPLC法同时测定复方磺胺甲噁唑颗粒剂中磺胺甲噁唑(SMZ)和甲氧苄啶(TMP)的含量。方法:色谱柱为ODS C_(18)柱,4.6x150mm,流动相为甲醇-磷酸盐缓冲液(pH5.90)(20:80),检测波长为240nm。结果:线性范围分别为:SMZ 20～181μg·ml^(-1),(r=0.9999);TMP 4～38μg·ml^(-1),(r=0.9999)。平均回收率分别为:SMZ100.5％(RSD=0.29％);TMP 100.3％(RSD=0.51％)。结论:本法分离度好,快速,简便,可同时测定该品中的两种组分。

Objective: To establish the simultaneous content determination of sulfamethoxazole(SMZ) and trimethoprim (TMP) in Compound Sulfamethoxazole Granules by HPLC.Methods:two components of canules and internal standand(caffeine)were separated on a reversed—phase ODS column by using a mobile phase containing a mixture of methanol and 0.05mol·L^(-1)phosphate buffer(pH5.90)(20:80),and detected at 240nm.Results:Linear calibration graphs were abtained for SMZ and TMP over the concentration ranges of 20～181μg·ml^(-1)(r=0.9999)and 4～38μg·ml^(-1),(r=0.9999)respectively.The avehage recovery(RSD,(n=9)was 100.5%(0.29%)and 100.3%(0.51%)vespectively.Conclusion:The method is high resolution,simple,rapid,precise for simultaneou determination two component,and better than method of Pharmacopoeia of the people's republic of China(1995).