Bcl-2蛋白在乳腺正常组织、良性病变及乳腺癌中表达的免疫组织化学研究

Immunohistochemical study of Bcl-2 protein overexpression in human breast tissues and breast neoplasms

目的 :通过检测Bcl- 2蛋白在 70例乳腺正常组织、乳腺良性病变及乳腺癌中的表达 ,探讨Bcl- 2对乳腺细胞增生、生存和凋亡的影响。方法 :应用S -P法抗Bcl- 2免疫组织化学染色 ,光镜观察。结果 :2 0例乳腺正常导管上皮、15例乳腺良性病变及 35例乳腺癌Bcl- 2蛋白阳性表达率分别为 95 0 %、80 0 %、5 1 4% ,三组Bcl - 2蛋白表达有显著性差异 (P <0 0 1)。结论 :Bcl- 2蛋白过表达与乳腺细胞增生、生存和凋亡有一定的关系。

Objective:To detect the expression of Bcl-2 protein in normal breast tissues and breast neoplasms so as to study its effects on proliferation,subsistence and death in breast cells.Methods:These tissues were stained with monoclonal anti-Bcl-2(S-P method)and observed by the microscope.Results:The positive expressions for Bcl-2 in normal breast epithelium,benign group and malignant group were 93 3%,80 0%,51 4%,respectively.There were significant differece in three groups(p<0 01).Conclusion:Overexpression of Bcl-2 protein might be related to proliferation,subsistence and death in breast cells.