产超广谱β-内酰胺酶细菌的检测

Determination of ESBLs-producing Bacteria

目的 :评价产超广谱 β-内酰胺酶 (ESBL s)细菌的 2种检测方法。方法 :16 9株大肠埃希氏菌和 73株肺炎克雷伯氏菌用复合纸片法做表型确证试验及双纸片协同法进行对照监测。结果 :确证法检测 ESBL s菌株阳性率为 18.6 % ,双纸片法阳性率为 13%。结论 :选择准确快速的检测方法 ,提高 ESBL s菌株的阳性检出率 。

Purpose To evaluate two different kinds of the tests for ESBLs-producing bacteria. Methods In this study 169 strains of Escherichia coli and 73 strains of Klebsiella pneumoniae were respectively cultured using both a compound paper disk method for phenotype confirmatory tests and a double paper disk method for synergistic tests. All these tests were under contrast monitoring. Results The positive rate of the ESBLs-produsing strains determined by phenotype confirmatory tests was 18.6 %,while the positive rate of ESBLs-produsing strains determined by the double paper disk method was 13 %. Conclusion Choosing an accurate and fast testing method for a higher detectable positive rate of ESBLs-produsing strains is of great clinical significance.