人工听骨周围组织细胞反应的实验研究

The study on the cellular responses around artificial ear ossicles in rats

为比较不同人工听骨植入组织后的局部组织细胞学反应 ,我们将氧化铝和羟基磷灰石人工听骨植入大鼠皮下组织 ,分别于术后不同时间取出包绕有人工听骨的周围组织并作成 6 μm薄切片 ,伊红染色 ,定量统计出组织中各炎症反应细胞数。发现两种材料在植入术后 3日内的反应细胞均以嗜中性粒细胞、巨噬细胞等急性炎症细胞为主 ,但它们的消退也非常迅速 ,至术后 14日时 ,组织细胞反应已与对照组基本无异。 14日之后的细胞反应既以纤维母细胞、纤维细胞为主 ,反映了植入物在组织中的稳定程度 ,但羟基磷灰石听骨材料实验组在植入后期有急性炎症细胞的再次出现现象。本实验还对两种材料的表面结构进行了扫描电镜观察。文中讨论了两种材料不同的理化性质 ,提示上述二种材料是生物相容性良好的成形手术材料 ,但不能忽视在组织中它们仍是作为一种异物存在 。

Objective: In order to study local histocellular responses after implanting synthetic ear ossicles made by aluminum oxide and hydroxyapatite respectively. Methods: There were 80 rats which were divided into two groups randomly. These two kind of artificial ear ossicles were implanted into the subcutaneous tissue of rats respectively. Forty rats were divided into experimental group (32 rats and divided into 8 groups) and control group (8 rats and divied into 4 groups). Tissues around artificial ear ossicles were observed and responsive cells were counted by optics microscopy after 1, 3, 7, 14, 30,90,180 and 300 days implantation. Results: Neutrophiles and macrophages predominated in two groups within initial three days after implantation, which had the significant differences with control group, but rapidly decreased later. Cellular reactions to artificial ear ossicles were similar to the reactions in control groups at 14 days. From 14 days, cells around implants were mainly fibroblasts and fibrocytes, which reflected the stability of artificial ear ossicles in tissues. However, acute imflammatory cells reappeared in later period of experiment in hydroxyapatite group. Conclusion: Experimental findings suggested that these two kinds of biosynthesis materials had satisfactory biocompatibility. But it could not be ignored that they were still foreign bodies to the tissues. Therefore, it is necessary to pay more attention to follow up, strengthen anti\|inflammation and anti\|rejection after implantation.