银杏叶提取液对缺血再灌注骨骼肌线粒体的保护作用

Protective effect of Ginkgo Biloba succi on mitochondria of skeletal muscle during ischemia and reperfusion injury

目的 观察银杏叶提取液对肢体缺血再灌注损伤的影响。方法 制作兔肢体缺血再灌注损伤动物模型 ,实验分对照组、再灌注组和治疗组。检测骨骼肌三磷酸腺苷 (ATP)水平并观察线粒体超微结构的变化 ,测定线粒体丙二醛 (MDA)、还原型谷胱甘肽 (GSH)和线粒体Ca含量。结果 再灌注组和对照组比较 ,以上各项指标差异显著 (P <0 0 1,P <0 0 5 )。银杏叶提取液促进骨骼肌能量代谢、维持线粒体结构完整性、降低线粒体MDA含量、提高线粒体GSH水平并抑制线粒体Ca超载。治疗组各项测定指标较再灌注组比较明显改善 (P <0 0 1,P <0 0 5 )。结论 银杏叶提取液对缺血再灌注骨骼肌线粒体有保护作用。

Objective To observe the effect of Ginkgo Biloba succi on ischemia and reperfusion injury of Limb Methods The experimental model of ischemia and reperfusion injury of limb was produced in rabbits which were divided into control group,reperfusion group and treatment group.The changes of ATP synthesis and mitochondrial ultrastructure were observed,while the contents of MDA,GSH and Ca in mitochondria were measured respectively.Results The indexes of reperfusion group changed significantly as compared with those of control group(P<0 01,P<0 05).Ginkgo Biloba succi promoted the recovery of energy metabolism,maintained the integrality in mitochondrial structure,reduced the mintochondrial MDA content,increased the level of GSH,inhibited the calcium overload.The indexes of treatment group improved significantly as compared with those of reperfusion group(P<0 01,P<0 05).Conclusion Ginkgo Biloba succi might have the protective effect on muscle cells during ischemia and reperfusion injury of limb.