三九胃泰颗粒对大鼠急性胃粘膜损伤的修复作用

Effect of Sanjiuweitai Granula on Acute Gastric Mucosa Lesion of Rats

目的 :观察三九胃泰颗粒对胃粘膜损伤修复和细胞增殖活性调控的影响。方法 :采用无水乙醇 1m l胃饲诱发急性胃粘膜损伤大鼠模型并用三九胃泰颗粒治疗 ,免疫组织化学技术检测胃上皮细胞的增殖改变 ,早期应答基因 c- jun和 c- met的表达。结果 :三九胃泰颗粒、胃舒颗粒、养胃冲剂及丽珠得乐治疗组大鼠胃粘膜损伤指数均显著低于无水乙醇模型组及自然恢复组 ,尤以三九胃泰颗粒治疗后为甚 (P <0 .0 5 ) ;免疫组织化学显示三九胃泰颗粒、胃舒颗粒、养胃冲剂及丽珠得乐治疗组大鼠胃粘膜增殖细胞核抗原标记的阳性细胞数量、c- jun和 c- met的阳性表达均有高于无水乙醇模型组及自然恢复组的趋势 ,尤以三九胃泰颗粒治疗后为甚 (P <0 .0 5 )。结论

Objective:To investigate the effect of Sanjiuweitai Granula(SG) on acute gastric mucosa lesion and cell proliferation activity.Methods:Rat model of acute gastric mucosa lesion was caused by feeding 1 ml ethanol and treated with SG.The expression of PCNA,c jun and c met were analyzed by immunohistochemical method.Results: Gastric mucosa lesion index(UI) of SG group,Weishu Granula(WG) group,Yangwei Granula(YG) group and Lizhudele group were significantly lower than those of model group and spontaneous recovery group, and the effect of SG was the most significant among these drugs(P< 0.05 ).The results of immunohistochemical staining revealed that the number of PCNA marked positive cell in gastric mucosa, positive expression of c jun and c met in SG group,WG group,YG group and Lizhudele group were higher than those in model group and spontaneous recovery group,and the effect of SG was the most significant among these drugs(P< 0.05 ).Conclusion:SG could accelerate the recovery of acute gastric mucosa lesion.