牛疱疹病毒1型洛精株gD基因的分子克隆及序列分析

The molecular cloning and sequence analysis of the glycoprotein D gene of bovine herpesvirus 1 Luojing strain

根据已发表的牛疱疹病毒 1型 (BHV 1)P8 2株 gD基因的核苷酸序列设计了 1对特异性引物 ,对我国BHV 1洛精株 gD基因进行PCR扩增 ,并对其克隆、测序和分析。结果表明 ,gD基因的核苷酸长度为 12 5 1bp ,其编码 417个氨基酸 ;BHV 1洛精株gD与国外报道的P8 2株的 gD基因的核苷酸的同源性高达 99.92 % ,氨基酸的同源性高达 10 0 %。这说明BHV 1的

By means of PCR,the gene encoding gD of bovine herpesvirus (BHV 1) Luojing strain was amplified,cloned and sequenced with a pair of specific primers designed according to the published nucleotide sequence of the gD gene of BHV 1 P8 2 strain.The nucleotide sequence of the gD gene was 1 251 bp long, encoding 417 amino acids.Comparied with the published P8 2 strain,the homology of the neccleotide sequence is 99.92%,and that of the deduced amino acid sequence is 100%.The results indicated that the gD gene of BHV 1 is highly conservative.