北京鸭脊髓灰质细胞构筑学研究

A STUDY ON THE CYTOARCHITECTURE OF THE SPINAL CORD IN BEIJING DUCK( ANSA DOMESTICUS L. )

取用4～6周龄北京鸭7例(雌雄不拘)分别做石蜡、冰冻切片,用甲酚固紫、甲苯胺蓝、美蓝和中性红染色。在光学显微镜下明视野观察。用测微尺测量细胞的长径和短径。结果如下:北京鸭脊髓的主要核团有:背角边缘核、胶状质核、背角固有核、背侧巨细胞核、Terni氏核、外侧运动核和内侧运动核。根据脊髓灰质的细胞形态特点,也可将北京鸭脊髓灰质划分为X个板层:背角头部由Ⅰ—Ⅳ层组成;背角颈部为V层,此层可突入到背角基部;Ⅵ层位于背角基部腹侧部和中间带背侧部分,此层只在脊髓膨大部明显;Ⅶ层占据中间带广阔的区域;Ⅷ层位于腹角的内侧部,也只在膨大部明显。Ⅸ层由腹角运动核构成。脊髓灰质内神经元的细胞体形态有圆形、三角形、梭形、多角形等。小型神经元的胞体为10～15×15～20μm;中型神经元胞体为15～20×20～35μm;大型神经元胞体在35μm以上。

Seven spinal cords were taken from 4-6 weeks old Beijing ducks for making sections stained with cresyl fast violet, toluidine blue, neutral red and methylene blue. The sections were observed under light field and measured size of the neural cell bodies. The results are as follow. The column of spinal cord in Beijing duck can be distingushed are columna medialis, columna lateralis, columna preganglionica Terni, columna dorsalis magnocellularis, columna dorsalis properi, columna substantia gelatinosa and columna dorsalis marginalis. According to the features of distribution of neural cells in the spinal cord in Beijing duck, we can divide the gray matter of spinal cord into ten lamella. The head of the corni dorsalis includes Ⅰ-Ⅳ lamella. V lamella lies in cervi of corni dorsalis. The Ⅵ lamella occupies basilar part of corni dorsalis, it is distingushed only in the two enlargements. The Ⅶ lamella situates in a extensive area of zona intermedialis. The ventro-medialis part of the corni ventralis is occupied by Ⅷ lamella, it's observed only in the two enlargements, too. The Ⅸ lamella is composed of columnae medialis and lateralis. The Ⅹ lamella occures in a round region surround canali centralis, The shapes of the neuron's bodies including round, oval, spindle, triangullaris and multipolaris. The small neurons are 10-15 × 15-20μm,the middle ones are 15-20×20-35μm, the magnoneurons are over 35μm.