烟雾吸入伤大鼠肺组织细胞凋亡与增殖的变化

The change in apoptosis and proliferation of pulmonary tissue cells in rats with smoke inhalation injury

目的 观察大鼠烟雾吸入性损伤后肺组织细胞凋亡、细胞增殖的变化规律 ,探讨肺组织损伤修复过程中细胞凋亡的意义。 方法 制作大鼠烟雾吸入性损伤模型 ,随机分为正常对照组、烟雾致伤组 ,应用TUNEL技术及免疫组化技术 ,观察伤后各时相点肺组织细胞凋亡指数及增殖细胞核抗原指数的变化。 结果 (1)烟雾吸入伤后细胞凋亡指数在伤后 2h增加并维持在高平台区。 (2 )增殖细胞核抗原指数在伤后 12h开始升高 ,3d时达到高峰 ,以后保持在较高水平。 结论 细胞凋亡不仅参与了烟雾吸入伤的早期肺损害 。

Objective To observe the rule of the change of apoptosis and proliferation of pulmonary tissue cells in rats with inhalation injury, so as to explore the significance of apoptosis in the repairing process of pulmonary tissue injury. Methods Smoke inhalation injury model was established in rats. The rats were randomly divided into normal control (NC) and smoke inhalation injury (SI) groups. TUNEL and immunohistochemistry methods were employed to determine the changes in cellular apoptotic and proliferating cell nuclear antigen (PCNA) indices of the pulmonary tissue at different postburn time points. Results (1) The apoptotic index increased at 2 postburn hours (PBHs) and remained at high levels thereafter. (2) The PCNA index increased at 12 PBHs , reaching top level at 3 postburn days (PBDs),remaining kept at relativly high level later. Conclusion Apoptosis not only played roles in the early pulmonary injury after smoke inhalation injury, but also participated in the repair and modification of the proliferated tissue during later reconstruction.