自体异体表皮细胞与自体淋巴细胞混合培养的免疫反应

Immune reaction in the mixed culture of host lymphocytes with allogenic and host epithelial cells

目的 探讨嵌入式混合植皮后排异反应受抑的免疫学机制。 方法 按不同比例 ,将烧伤患者表皮细胞、淋巴细胞与异体表皮细胞混合培养 ,模拟混合植皮时自体皮岛的局部免疫微环境 ,另将正常人相应细胞混合培养 (对照 )。采用3 H TdR法检测淋巴细胞cpm值 ,采用免疫组化技术检测HLA分子和T细胞亚群。 结果 (1)烧伤患者表皮细胞能有效抑制淋巴细胞增殖反应 ,而正常人无此现象 ;(2 )烧伤患者表皮细胞中HLA DQ分子不介导自体淋巴细胞增殖受抑 ;(3)烧伤患者表皮细胞中HLA DR表达阳性率明显高于正常人 (P <0 .0 5 ) ,两者淋巴细胞中HLA DR分子表达相比较 ,差异无显著性意义 (P >0 .0 5 ) ;(4)烧伤患者淋巴细胞中CD8表达明显高于正常人 (P <0 0 1) ,CD4表达明显低于正常人 (P <0 .0 1) ,CD3表达与正常人相比差异无显著性意义 (P >0 0 5 )。 结论 大面积烧伤患者混合植皮时 ,自体表皮细胞可明显抑制淋巴细胞的增殖反应 。

Objective To observe the immune reaction in the mixed culture of host lymphocytes with allogenic and host endothelial cells. Methods The host epithelial cells and lymphocytes from burn patients and allogenic epithelial cells were mix cultured in different ratios, so as to simulate the local immune micro environment of host skin island in intermingled skin grafting. In addition, the cells from normal human subjects were also mix cultured as control. The lymphocyte cpm values were detected by 3 H TdR and HLA molecules and T cell subgroup were determined by immunohistological technique. Results (1) The lymphocyte proliferation reaction could be effectively inhibited by the epithelial cells from burn patients but not from normal control.(2) The inhibition of host lymphocyte proliferation could not be mediated by the HLA DQ molecules of epithelium from burn patients. (3) The positive expression rate of HLA DR of epithelia from burn patients was evidently higher that that from normal control (P<0.05), (4) The CD8 expression of lymphocyte in burn patients was significantly higher than that in normal control (P<0.01), while the CD4 expression in burn patients was lower than that in normal control (P<0.01). But there was no obvious difference of the CD3 expression between patients and normal subjects (P>0.05). Conclusion The lymphocyte proliferation reaction could be obviously inhibited by the host epithelium, which might be related to the specific immune state of the host lymphocytes and epithelium of burn patients.