烫伤大鼠垂体前叶P物质和降钙素基因相关肽神经的变化

The changes in the morphology and distribution of substance P and calcitonin-gene related peptide nerves in the anterior pituitary of scalded rats

目的 观察烫伤早期大鼠垂体前叶P物质 (SP)与降钙素基因相关肽 (CGRP)神经的形态分布变化。 方法 采用大鼠 30 %TBSAⅢ度烫伤模型 ,于伤后 1、2、6、10、12、2 4、72h 7个时相点 ,对垂体前叶SP和CGRP两种肽能神经行免疫组织化学染色观察 ;经图像分析测定 ,比较两种神经覆盖面积的变化。 结果 两种肽能神经的形态变化明显 ;伤后 1hSP、CGRP神经数目与对照相比分别增加 85 .7%和 6 0 .5 % ,神经纤维的膨体、分枝等明显增多 ;2h后减少 ,2 4h后趋于恢复。图像分析显示 :两种肽能神经的覆盖面积呈正相关变化 ,其基本变化趋势为伤后 1h升高 ,随后降低 ,伤后 12h降至最低点 ,然后回升。 结论 烫伤后早期大鼠垂体前叶的SP与CGRP神经形态分布发生明显变化 。

Objective To observe the changes in the morphology and distribution of substance SP and calcitonin gene related peptide nerves in the anterior pituitary of scalded rats. Methods The rats inflicted 30% TBSA degree scalding of the back were employed as the model.The SP and CGRP peptide nerve fibres in the anterior pituitary were observed by immunohistochemistry staining during different postburn time points:1,2,6,10,12,24 and 72 postburn hours (PBHs).The areasof coverage of SP and CGRP nerve fibers were determined respectively in the anterior pituitaries by image analysis. Results The morphology of the two peptide nerve fibers exhibited obvious changes under microscope.The numbers of SP and CGRP nerve fibers increased by 85.7% and 60.5%,respectively at 1PBH when compared with those in control group.Moreover the varicosities and branches of the nerve fibers in scalded rats increased obviously at 1 PBH and decreased sharply at 2 PBH,bouncing back to near normal level at 24 PBH.reaching lmage analysis revealed that the changes in the areas of coverage of two peptide nerve fibers were correlated positively with each other.The basic trends of the changes were an increase at 1 PBH,a decrease thereafter,reaching the lowest level at 12 PBH with a rebound later. Conclusion The morphology and distribution of SP and CGRP nerve fibers of anterior pituitary in scalded rats exhibited obvious change after injury.Therefore,they might be involved in the functional modulation of systemic stress.