小鼠红白血病细胞在半相合型小鼠体内的生物特性

Biological Characterization of Mouse Erythroblastic Leukemia Cells in Haploidentical Mice

把可移植性EL96 11H 2d小鼠红白血病细胞 ,接种给 (BALB/c×C5 7BL/ 6 )F1H 2d/b代 (CB6F1)小鼠并连续在CB6F1传代 ,观察红白血病细胞在半相合型小鼠体内的生物特性 ,为骨髓或造血干细胞移植中研究和观察移植物抗白血病 (GVL)作用提供实验对象。把EL96 11H 2d小鼠脾细胞经尾静脉接种给CB6F1H 2d/b小鼠 ,再把红白血病细胞在CB6F1传代接种 ,建立F1代红白血病模型。结果表明 :每只鼠在静脉接种 10 3 - 10 8个白血病细胞的情况下 ,发病率 10 0 % ;所接种的白血病细胞数量与存活时间呈线性关系 ;接种 10 6细胞的小鼠平均生存时间为 ( 9.6± 0 .8)天。在CB6F1体内连续传 4代 ,发病率 10 0 %。白血病细胞主要侵及肝、脾和骨髓组织 ,血红蛋白染色呈阳性 ,过氧化物酶反应阴性 ,对阿糖胞苷和环磷酰胺等化疗药物敏感。结论 :利用实验室常用的C5 7BL/ 6×BALB/c小鼠建立F1代小鼠红白血病模型 。

Using tranplantable erythroblastic leukemia cells of EL9611 H 2d , the cells were inoculated to CB6F 1 H 2d/b generation of BALB/c×C57BL/6 mouse, the biological characterization of erythroblastic leukemia in haploidentical mouse was studied, that provides an experimental model for the study of graft versus leukemia(GVL) with bone marrow or stem cell transplantation. When 10 3-10 8 of the spleen cells of EL9611 H 2d had been intravenously inoculated to CB6F 1 mouse, the erythroblastic leukemia cells were transplanted successively and the F 1 generation of erythroblastic leukemia model in mice was established with 100% incidence of erythroblastic leukemia. There was a linear relationship between the survival time and the number of leukemc cell. The survival time of the mice was (9.6±0.8) days when 10 6 cells were inoculated. If the CB6F 1 mouse was transplanted successively for four generations, the incidence was 100%. The main targets for the leukemic EL9611 H 2d cells were liver, spleen and marrow. The reaction of the erythroblastic leukemia cells for hemoglobin staining was positive, while the peroxidase reaction was negative. These cells were sensitive to some chemotherapeutic drugs, such as cytosine arabinoside and cyclophosphamide. This study presents the convenience for the studies on the GVL with haplo allogeneic transplantation, in the F 1 generation of erythroblastic leukemia model of the commonly used CD57BL/6×BALB/c mouse.