94株肠产毒性大肠杆菌中耶尔森菌强毒力岛的检测

Detection of yersinia high pathogenicity island in 94 enterotoxigenic escherichia coli strains

目的 检测 94株肠产毒性大肠杆菌中耶尔森菌强毒力岛。方法 对分离自中国腹泻病人的肠产毒性大肠杆菌 ,采用 PCR和 DNA打点杂交的方法 ,检测耶尔森菌强毒力岛核心区的 irp8、irp 2、irp 3、irp 4、irp 5及 fyu A基因。结果 分离的肠产毒性大肠杆菌 ,14 % (13/94 )的菌株 irp8、irp 2、irp 3、irp 4、irp 5及 fyu A基因扩增阳性 ,以 irp 2及 fyu A为探针进行 DNA打点杂交 ,上述菌株均为阳性。结论 14

Objective To detect Yersinia high pathogenicity island (HPI) in enterotoxigenic E. coli. Methods With PCR and DNA dot hybridization, ninety four enterotoxigenic E. coli (ETEC) strains isolated from patients with diarrhea in China were investigated for the presence of Yersinia HPI genes. Results The Yersinia HPI genes such as irp 8, irp 2, irp 3, irp 4, irp 5 and fyuA were amplified in 14%(13/94) of the strains by PCR, and for the PCR positive strains, DNA dot hybridizations with probes for irp 2 and fyuA genes were also postive. Conclusions 14% of the ETEC strains in China contain Yersinia HPI.