摘要
采用PCR检测方法从饲料的主要原料豆粕、玉米蛋白粉中成功地检出启动子 35S (35S -promoter ,origi natedfromcauliflowermosaicvirus)、终止子NOS (nopalinesynthase -terminator,derivedfromAgrobacteriumtumefa ciens)、耐除草剂基因EPSPS(5 -enolpyruvylshikimate - 3-phosphatesynthase)和抗虫基因CryIA(b) (delta -endo toxin ,evolvedfromBacillusthuringiensissubsp .kurstaki)等转基因成分 ,并通过扩增玉米自身蛋白基因Zein(apro teinextractedfromcorngluten)及大豆自身基因Lectin(chitin -bindingprotein)的引物和阴阳性对照、阴阳性质控 ,避免假阳性、假阴性结果。该方法已在口岸进口饲料原料转基因检测中得到初步应用。
Based on the heterogenous genes usually used in transgenic crops,the PCR technique was performed with primers derived from CaMV 35S promoter(35S-promoter,originated from cauliflower mosaic virus), NOS terminator(nopaline synthase-terminator,derived from Agrobacterium tumefaciens),EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene,and CryIA(b) (delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)gene to detect transgenic agents from feed raw materials of soybean dregs and corn gluten meal,respectively.Endogenous corn Zein (a protein extracted from corn gluten) gene,soybean Lectin (chitin-binding protein) gene and negative,positive control were applied for avoiding false results.The method established here has been succeessfully applied in detecting transgenic elements in imported feed raw material.
出处
《遗传》
CAS
CSCD
北大核心
2002年第3期293-296,共4页
Hereditas（Beijing)
基金
中国国家出入境检验检疫局 2 0 0 0年科学研究与技术开发项目 (K0 40 -2 0 0 0 )
