荧光原位杂交技术(FISH)及其在环境微生物学中的应用

The process of FISH and its applications in the environmental microbiology

微生物对整个生态系统具有重要的影响,了解和检测微生物的种类具有十分重要的意义。但是传统的培养方法又不能满足科学研究的需要,荧光原位杂交技术(ＦＩＳＨ)就是近年发展起来的一种快速准确检测微生物的方法。作者较全面地介绍了ＦＩＳＨ技术的优点、微生物的分离和固定方法,以及原位杂交过程,同时对ＦＩＳＨ技术在环境微生物学中的应用及其前景作了展望。

Microorganisms have important influences on the whole ecosystem; understanding and detecting the species of microorganisms are very necessary. But traditional culture method can't meet the requirements of the development of science. In the paper, the advantages, separating and fixing of microorganisms, the process of hybridization in situ, together with the future applications of FISH in the environmental microbiology are introduced.