欧洲鳗鲡外周血细胞的显微和超微结构

MICROSTRUCTURE AND ULTRASTRUCTURE OF THE PERIPHERAL BLOOD CELLS OF EUROPEAN EEL(ANGUILLA ANGUILLA)

利用光镜和透射电镜技术研究了欧洲鳗鲡 (Anguillaanguilla)外周血细胞的显微和亚显微结构。结果表明 :在外周血细胞中可区分出红细胞、单核细胞、大淋巴细胞、小淋巴细胞、嗜中性粒细胞和血栓细胞 ;嗜中性粒细胞内的特殊颗粒包括A、B、C三型 ;嗜中性粒细胞分为Ⅰ型粒细胞 (内含A、B、C三种特殊颗粒 )、Ⅱ型粒细胞 (内含A型和C型两种特殊颗粒 )和Ⅲ型粒细胞 (内含C型特殊颗粒 )。还见到幼稚的和正在分裂的红细胞和单核细胞 ,提示幼稚的红细胞能直接进入外周血流中。嗜酸性粒细胞和嗜碱性粒细胞无论在血涂片上或超薄切片上均未见到。描述了上述各种血细胞在光镜和电镜观察下的形态和细微结构。血栓细胞体积最小 ,嗜中性粒细胞体积最大 ;淋巴细胞数目最多 ,嗜中性粒细胞数目最少。

We studied the peripheral blood cells of one\|year\|old European eel( Anguilla anguilla )using light microscopy and transmission electron microscopy.For morphology,peroxidase Giemsa was used.The morphological study revealed that the peripheral blood has the following cell types: erythrocyte, neutrophil, lymphocyte and thrombocyte. Erythrocytes are 11 82±0 75 μm in length and 7 84±0 83 μm in width,oval shape and contain oval nuclei and dark gray cytoplasm.They were almost completely devoid of organelle,except for a few vesicles and small mitochondria.Neutrophils were 11 73±1 45 μm in length and 10 73±1 14 μm in width,round or oval,containing round,bilobed or trilobed nuclei and whitish cytoplasm.There were three special granules in neutrophils. Special granules A were round or oval shape,possess thread\|like,cotton\|like or crystalline structrures,special granules B were long oval or rod\|like,dark stained and possess cotton\|like structures,which paralleled to the long axia.Special granules C were round or oval,possessed light gray structures.The ultrastructural study revealed that the neutrophils include three types of cells according to their special granules(A,B and C special granules):type Ⅰ(included A,B and C special granules)、type Ⅱ(included A and C special granules)and type Ⅲ(included C special granules).A greater amount of mitochondria,small tubules which resemble smooth endoplasmic reticulum,and numerous oval on elongated granules which contain a striated or crystalline structure,were observed in neutrophils although neutrophils are similar to monocytes in size and shape.Monocytes were of 10 78±1 55 μm in length and 9 74±1 75 μm in width, containing round or oval nuclei.A greater amount of vesicles and a few mitochondria were observed.Thrombocytes of 1 97±0 71 μm in length and 4 02±0 74 μm in width,were round,oval,spindle or tadpole\|like shape.Deep indentations of the plasmalemma,which gave appearance of vacuoles,were often seen.Lymphocytes were clearly different from thrombocytes by the presence of large number of free ribosomes.Neither eosinophils nor basophils were found directly in peripheral blood.The nuclear division of erythrocyte and monocyte were observed in peripheral blood indicating that erythroblasts and young monocytes could enter the peripheral blood.The size of thrombocyte was the smallest while that of the neutrophil was the largest and the number of neutrophils was the least while that of lymphocytes was the most in the blood cells.