腐生菌对水解聚丙烯酰胺降解过程的研究

STUDY ON BIODEGRADATION OF PARTIALLY HYDROLYZED POLYACRYLAMIDE BY TOTAL GROWTH BACTERIA

水解聚丙烯酰胺被广泛应用于油田以提高原油的采收率。在实验室模拟油田的二元复合聚合物驱的条件研究腐生菌(简称TGB)在碱-聚合物驱中的生长情况,讨论改变溶液pH值、TGB初始接种量、培养时间、连续活化次数对水解聚丙烯酰胺(HPAM)溶液粘度的影响。研究表明,连续活化5次的TGB在1000mg/L的HPAM中恒温培养7d,可使溶液粘度损失率达11.2％。通过SPS100型高效液相色谱仪测接种TGB前后HPAM的相对分子质量及其分布,探讨HPAM溶液粘度的损失机理,初步认为是由于在TGB的作用下,HPAM的高分子链断裂发生生物降解所致。

Hydrolyzed polyacrylamide (HPAM) has been widely used in oil field for boosting the crude oil recovery ratio. The growing of total growth bacteria (TGB) in partially hydrolyzed polyarcylamide was investigated in the laboratory. The effects of pH, initial inoculated bacteria number, culturing time and continuous, activating times on the viscosity of HPAM were discussed. The experimental results showed that the viscosity loss of 1000mg/L HPAM solution reached 11.2％ after 5 times activation of the TGB in 7 days cultivation under 30℃. The molecular weight and molecular weight distribution of HPAM in prior and after inoculation were detected by employing SP8100 liquidjelly chromatography. The experimental results also showed that the mechanism of the viscosity loss of HPAM was attributed to the biodegradation of the high molecular HPAM.