不同发育时期小鼠胚泡表面Lewis寡糖抗原的表达

Analysis of Expression of Lewis Oligosaccharide Antigens on Mouse Embryos

在胚泡表面表达的Lewis寡糖抗原 (LewisX ,LewisY)在胚胎发育以及着床过程中起重要作用 .应用免疫印迹和免疫荧光等方法对着床前小鼠胚泡表面的Lewis寡糖抗原进行分析 .结果发现 :小鼠胚泡LewisX寡糖蛋白有 2 7kD、2 9kD、6 8kD和 80kD 4种 ,LewisY寡糖蛋白有 70kD和 90kD 2种 ;2种寡糖抗原均在 8细胞时期开始表达 ,其中 ,LewisY寡糖抗原在胚泡表面的表达持续升高 ,直至胚泡着床 ;而LewisX寡糖抗原的表达则在桑椹期后逐渐降低 ,但仍在胚胎期的囊胚腔侧的顶端可见有部分表达 ;应用RT PCR的分析结果显示 ,LewisX合成的关键糖基转移酶FUT9基因在 4细胞及桑椹期高表达 ,到胚泡期虽然强度明显减弱 ,但仍有表达 ;而LewisY合成关键酶FUT1基因在 4细胞未见表达 ,在桑椹和胚泡阶段均有表达并逐渐升高 ,表达趋势与相应寡糖的表达趋势基本一致 .结果说明 。

Lewis oligosaccharide antigens (Lewis X, Lewis Y) which were expressed on embryonic surface play important roles during embryogenesis and implantation. The immuno fluorescence,Western blotting and RT PCR were used for analysis of Lewis oligosaccharide antigens on cell surface during mouse embryogenesis. Western blotting showed that four different Lewis X (approximately 27 kD,29 kD,68 kD and 80 kD) and two Lewis Y (approximately 70 kD and 90 kD ) oligosaccharide antigens were expressed in embryos. Both of them occurred at the 8 cell stage, but Lewis X antigens got their maximum expression at the murola stage, then declined and kept expression on limited area of blastocyst. Lewis Y antigens continuously increased and kept maximum expression till implantation stage. The gene of the key enzyme FUT9 for synthesis of Lewis X oligosaccharide was continuously expressed during embryogenesis, although at blastocyst stage, its expression decreased; and the expression of the key enzyme FUT1 for synthesis of Lewis Y oligosaccharide was increased from the morula stage, and kept in high level till implantation. The results suggest that expression of Lewis antigens on mouse embryo surface is controlled by transcriptation of corresponding glycosyltransferase genes.