微量材料系列性基因表达分析技术的研究

Serial Analysis of Gene Expression From Minimal Amount of RNA Material

建立了微量材料 (1μgRNA)系列性基因表达分析技术 (minimalserialanalysisofgeneexpression ,miniSAGE) ,并用miniSAGE技术对冠心病 (coronaryarterydisease ,CAD)患者和正常人成纤维细胞的基因表达谱进行了分析 ,合成了两个高质量的SAGE库 ,比较了两个库的SAGE标签 ,为CAD发病机理的研究提供了大量表达异常基因的资料 .从而为后续分离与CAD发病相关的新基因打下了基础 .miniSAGE技术是一种有力的基因表达分析方法 ,可用于微量RNA (1μg)材料的基因表达分析 。

A miniSAGE (minimal serial analysis of gene expression) technique was established for determining the gene expression pattern from minimal amount of starting material (1 mug RNA). The gene profiles of two fibroblast cell lines were analyzed. One was from a coronary artery disease (CAD) patient, another one was from a normal person. Two high quality SAGE libraries were synthesized. Comparing the SAGE tags in the two libraries, abounding data about the differential expression genes was provided for the research on the mechanism of CAD. The new gene related with CAD could be isolated based on these data. The miniSAGE technique is a powerful gene expression analysis tool for study on complex disease, and can be applied on only 1 mug RNA.