人鼻咽癌单克隆细胞株在传代移植的裸鼠体内转移与HLA表达定量的关系

METASTASIS AND HLA EXPRESSION IN TRANSPLANTED MONOCLONE CELLS OF HUMAN NASOPHARYNGEAL CARCINOMA IN NUDE MICE

为了研究人鼻咽癌单克隆细胞裸鼠体内传代转移特性及 HL A的表达变化 ,我们以人鼻咽癌单克隆细胞株 (CNE-2 Z- H5 )为对象 ,将各单克隆细胞株移植于裸鼠 ,待肿瘤发生转移后 ,取其淋巴结和肺转移灶癌细胞再次移植裸鼠 ,如此移植 2 - 4代 ,观察每代裸鼠的转移特性及鼻咽癌细胞 HL A表达的免疫组织化学定量变化。结果显示随着鼻咽癌单克隆细胞的传代移植 ,每代裸鼠肿瘤的转移率增高 (P<0 .0 1) ,转移途径更为单一。淋巴道转移的克隆细胞其 HL A 类抗原的表达随体内传代次数的增加而呈明显降低的趋势 ,与原代比较有显著性差异 (P<0 .0 1) ,但第 4代又急刷升高 (P<0 .0 1) ;HL A 类抗原的表达则呈波浪样改变 ;各代间表达平均强度变化不规则。肺转移灶癌细胞的 HL A 、 HL A 类抗原表达明显降低 ,两者与原代癌细胞比较都有显著性差异 (P<0 .0 1)。表明鼻咽癌细胞演进中转移能力和细胞本身特性、体内的环境选择关系密切 ,且可能和癌细胞 HL

To study the characteristics of metastasis and HLA expression in transplanted of human nasopharyngeal carcinoma, the monoclone cells of nasopharyngeal carcinomas CNE 2Z H5 were inoculated into nude mice The lymph nodes and lung metastatic foci of transplanted tumors were transplanted again into other nude mice After 2 4 cycles the metastatic phenotype and the expression of HLA were detected in every generation of transplanted tumors The results showed that the metastatic rate was lower in the last passage of transplanted tumor than in the first ( P <0 01) and the metastatic paths became unitary The HLA antigen class Ⅰ expression declined in all passage of transplanted tumors in lymphnodes ( P <0 01) but significantly increased sharply in the fourth passage ( P <0 01), while the class Ⅱ expression changed irregularly The expression of HLA antigen class Ⅰ and class Ⅱ in cells of lung metastatic tumors were obviously decreased ( P <0 01) The metastatic ability of human nasopharyngeal carcinoma transplanted in vivo may be closely related with its characteristic the selection of environment, and the changes in HLA expression.