川芎嗪、丹参和地塞米松对大鼠肺气肿碱性成纤维细胞生长因子mRNA表达的影响

EFFECT OF LIGUSTRAZIN,LAVIA MILTIORRHIZA AND DEXAMETHASONE ON THE EXPRESSION OF BASIC FIBROBLAST GROWTH FACTOR mRNA IN RAT EMPHYSEMA

采用免疫细胞化学及原位杂交方法观察川芎嗪、丹参和地塞米松对木瓜蛋白酶所致大鼠肺气肿形成中肺组织碱性成纤维细胞生长因子 (b FGF) m RNA表达和肺泡 型上皮细胞增殖细胞核抗原 (PCNA )表达的影响。 Wistar大鼠随机分为正常对照、肺气肿 7天、 15天、 30天、川芎嗪、丹参和地塞米松治疗组 ,共 7个组。用一次气管内注入木瓜蛋白酶复制大鼠肺气肿模型 ,取肺组织作 b FGF m RNA原位杂交 ,分离肺泡 型上皮细胞作 PCNA免疫组织化学染色 ,用图像分析定量。结果显示注药后第 7天大鼠肺组织 b FGF m RNA表达至高峰 ,第 15天后表达逐渐减少 ,川芎嗪、丹参和地塞米松治疗 30天组 b FGF m RNA表达减少 ,但仍高于正常对照组 (P<0 .0 1)。肺泡 型上皮细胞 PCNA表达与肺组织 b FGFm RNA表达呈正相关 (r=0 .78,P<0 .0 1)。川芎嗪治疗组 PCNA表达明显降低 ,但仍高于正常对照组。

The effect of ligustrazin, salvia miltiorrhiza and dexamethasone on the expression of basic fibroblast growth factor (bFGF) mRNA in lung tissues and that of proliferating cell nuclear antigen (PCNA) of type Ⅱ alveolar cells during papain induced rat emphysema was studied with immunohistochemical and in situ hybridization techniques. The animals were divided into 7 groups: normal control group, untreated emphysema groups (7?15?30d), group treated 30d with Ligustrazin, Salvia Miltiorrhia and dexamethasone. The rat model of emphysema was induced by a single intratracheal instillation of papain. The results of bFGF mRNA in situ hybridization in rat lung tissues and PCNA of the type Ⅱ alveolar cells were quantified by a feature analysor. Immunohistochemical reaction was applied to PCNA expressions in type Ⅱ alveolar cells. In untreated emphysema groups bFGF mRNA reached the maximum on day 7 and gradually decreased on day 15. In all of treated groups, bFGF mRNA expression decreased ( P <0.01), but was still above normal level. There was a positive correlation between the expression of bFGF mRNA in rat lung tissue and the expression of PCNA in type Ⅱ alveolar cells ( r =0 78 P <0 01). In Ligustrazin group the PCNA expression in type Ⅱalveolar cells was obviously reduced, through still above normal level. The results suggest that Ligustrazin played an important roles in the therapy of emphysema.