在HSVI感染细胞中类PEBP蛋白基因的克隆及分析

Cloning and Analyzing a New PEBP Like Protein Gene in HumanFibroblast Cell Infected by HSV I

在细胞对病毒感染后的基因反应研究中 ,从克隆的HSVI感染后细胞特异cDNA文库中筛选出一个 70 5bp克隆基因—HSP 714,基因测序分析表明为一新的全基因序列 (GeneBankAccessionNumber:AF372 6 2 4) ,含有完整的ORF框架 ,编码 12 6个氨基酸 ;信息生物学分析结果表明该蛋白含有磷脂酰乙醇胺结合蛋白 (PEBP)结构域 ,与植物、哺乳动物等多物种类CEN家族产物有高度的同源性 ,此外该基因的进化分析结果表明此基因更接近于植物的类CEN家族。据此推测 。

In our study on the gene response to virus infection,a colony of 705bp-HSP-714 was selected from the cell specific cDNA library induced by HSVI infection.The gene sequence analysis revealed that it was a new complete gene sequence (Gene Bank Accession number:AF372624) with a complete ORF encoding 126 amino acids.The biological informatics analysis demonstrated that this protein contained a phosphatidylethanolamine-binding protein(PEBP) like domain,which had a high homology to the CEN-like family of plant and mammalian etc.genome.Furthermore,the evolution tree demonstrates that this gene shows more homologous to CEN-like family of plant.Therefore,it can be predicted that this gene might be a new member of human PEBP-like family with special significance.