高效液相法测定加替沙星尿药浓度

High Pressure Liquid Chromatography in detection of Gatifloxacin concentration in rat urine

目的 建立反相高效液相色谱法以测定大鼠尿液中加替沙星浓度。方法 尿液样品用二氯甲烷提取 ,以环丙沙星为内标 ,色谱柱 :LichrosorbC1 8,流动相 :甲醇 - 0 .0 1mol/L ,磷酸二氢钾缓冲液 - 0 .5mol/L ,四丁基氢氧化铵溶液 (1 5∶75∶1 .4) ,磷酸调 pH至 2 .6 ;流速为 1 .0ml/min ;检测波长 2 98nm。 结果 本法在 3 .1 0～ 1 55 .2 μg/ml范围内线性良好 ,r=0 .9995 ,日内RSD为 5 .1 %～ 9.7% ,日间RSD为 5 .7%～ 1 0 .2 % (n =5) ;尿液最低检测浓度为 50ng/ml。 结论 本法测试快速、准确。

Objective To establish a reversed phase High Pressure Liquid Chromatography (HPLC) Method in determination of Gatifloxacin concentration in rat urine. Methods The urine samples were extracted by dichloromethane solvent. Column: Lichrosorb C 18 ; mobile phase: mehthanol-0.01mol/L, potassium dihygrogen phosphate- 0.5mol/L, tetra-butylammiun hydroxygen (15∶75∶1.4), pH was adjusted to 2.6 with phosphoric acid; flow rate: 1.0 ml/min; detection wave length: 298nm. Results Assay linearity was obtained in the range of 3.10～155.2μg/ml, r=0.9995, intra-day and inter-day RSD were 5.1%～9.7% and 5.7%～10.2% (n=5), respectively; the lowest detectable concentration was 50ng/ml. Conclusions This method is rapid, accurate and good in reappearance.