子宫内膜异位症患者腹腔液对正常NK细胞活性的抑制及相关因素分析

Effect of endometriosis peritoneal fluid on natural killer cell activity of normal people

目的 :探讨子宫内膜异位症 (EM)患者的腹腔液对自然杀伤 (NK )细胞的作用 ,以及与腹腔液中白细胞介素 6 (IL -6 )、前列腺素 (PG)含量的关系。方法 :以 He L a细胞作为靶细胞 ,采用 L DH释放法测定与 EM患者腹腔液共同孵育后正常人血 NK细胞活性的变化。应用放射免疫法测定腹腔液中 PG和 IL - 6的含量。 结果 :在作用 2 h时 ,非 EM患者和 EM患者腹腔液对正常人外周血 NK细胞活性的抑制率分别为 (37.1± 18.9) %和 (6 5 .2± 2 1.8) % ,两者相差非常显著 (P<0 .0 1) ,抑制率与 EM临床分期呈正相关 (r=0 .6 0 2 3,P<0 .0 5 )。延长腹腔液与淋巴细胞的作用时间 ,则对 NK细胞活性的抑制率也随之升高。 EM - 期的患者腹腔液中 IL - 6含量为 (0 .6 1± 0 .4 7)μg/ L ,明显高于对照组的 (0 .4 3± 0 .39)μg/ L (P<0 .0 1) , - 期组 [(0 .4 9± 0 .36 )μg/ L ]与对照组相似 (P>0 .0 5 )。腹腔液中 IL - 6的含量与其对 NK细胞活性的抑制率之间无明显的相关性 (r=0 .2 38,P>0 .0 5 )。 EM患者腹腔液中 PGE2 和 PGF2α含量均明显升高 ,其中 - 期患者腹腔液对 NK细胞抑制率与PGE2 含量呈正相关 (r=0 .5 13,P<0 .0 5 ) ;与 PGF2α也呈正相关 (r=0 .4 2 5 ,P<0 .0 5 )。结论 :EM患者的腹腔液对

Objective:To investigate the effect of the peritoneal fluid (PF) in women with endometriosis (EM) on the natural killer (NK) cell activity, and to study its relationship with the level of prostaglandins(PGs) and interleukin 6(IL 6). Methods:HeLa cells were taken as target cell, the LDH releasing assay was used to measure NK cell activity after preincubation with PF of EM. The concentration of IL 6, PGE 2 and PGF 2α was measured by radioimmunoassay. Results:After incubated with PF of women with and without EM for 2 h, the inhibition percentages was(65.2±21.8)% and (37.1±18.9)% respectively ( P < 0.01). The inhibition percentage was correlated with the severity of EM( r =0.602 3, P <0.05). NK cell activity decreased significantly when prolonged the incubation time. The concentration of IL 6 in women with mild EM increased significantly compared with women without EM. The concentration of PGE 2 and PGF 2α increased significantly in women with endometrosis, and this was correlated with the suppressive effect on NK activity in women with minimal to mild EM. Conclusion:PF of EM can significantly decrease NK cell activity in vitro , the suppressive effect is correlated with the concentration of IL 6 of PF in women with minimal to mild EM.