神经肽Y对人血管平滑肌细胞脂质代谢的调制作用

The Changes of Lipid Metabolism in Human Vascular Smooth Muscle Cells Induced by Neuropeptide Y

为探讨神经肽Y在血管平滑肌细胞中对脂质代谢是否具有调制作用 ,以成人血管平滑肌细胞体外培养模型为研究对象 ,经不同浓度的神经肽Y作用后 ,运用激光扫描共聚焦显微镜和免疫荧光组织化学技术 ,定量观察神经肽Y对人血管平滑肌细胞的低密度脂蛋白受体表达的影响。结果发现 ,对照组人血管平滑肌细胞的低密度脂蛋白受体表达的平均荧光强度为 2 4 4 3± 1 89,而在浓度为 1 0 8mol L、1 0 7mol L、1 0 6mol L和 1 0 5mol L的神经肽Y作用下 ,各组的平均荧光强度分别为 2 0 1 5± 1 64、1 890± 1 4 5、1 841± 1 2 3和 1 786± 1 36 ,与对照组相比分别下降了1 7.49%、2 2 .62 %、2 4 .64 %和 2 6 .92 % (P <0 .0 1 ) ,且各浓度组之间多有显著差异 ,呈现一定的剂量依赖效应。结果提示 ,神经肽Y很可能通过下调人血管平滑肌细胞低密度脂蛋白受体的表达 ,从而影响血管平滑肌细胞的脂质代谢。

Aim To explore the effect of neuropeptide Y on lipid metabolism in human vascular smooth muscle cells. Methods The human vascular smooth muscle cell culture model was used for experiments. The expression of low density lipoprotein receptor in cultured human vascular smooth muscle cells were quantitatively assayed by the immunofluorescent staining under laser scanning confocal micropscope. Results The averaged fluorescent value in control group was 2443±189,while the values in neuropeptide Y groups were 2015±164,1890±145,1841±123 and 1786±136,decreasing by 26.92%, 24.64%, 22.62% and 17.49%(P<0.01),respectively in 10 8 mol/L, 10 7 mol/L, 10 6 mol/L and 10 5 mol/L concentrations of neuropeptide Y, compared with control group. There was significant differences among the different concentrations neuropeptide Y groups in a dose dependent manner. Conclusion Neuropeptide Y may influence the lipid metabolism of human vascular smooth cells by downregulating low density lipoprotein receptor in human vascular smooth muscle cells.