多沙唑嗪抑制大鼠血管平滑肌细胞增殖的实验研究

Inhibition of Doxazosin on Proliferation of Rat Vascular Smooth Muscle Cells

为探讨多沙唑嗪对大鼠血管平滑肌细胞增殖的抑制作用 ,将不同浓度的多沙唑嗪作用于体外培养的血管平滑肌细胞 ,采用氚 胸腺嘧啶核苷 (3 H TdR )掺入的方法检测平滑肌细胞的增殖。结果发现 ,多沙唑嗪能够抑制血管平滑肌细胞和不表达α1 受体的成纤维细胞的增殖。用不可逆的α1 受体阻滞剂酚苄明处理平滑肌细胞 ,多沙唑嗪仍表现为抑制增殖的作用。以上提示 ,多沙唑嗪对大鼠血管平滑肌细胞增殖的抑制作用与其对α1

Aim To investigate the effects of Doxazosin on the proliferation of rat vascular smooth muscle cells (VSMCs) cultivated in vitro. Methods Various concentrations of Doxazosin were added to the cultured rat VSMCs. 3 H TdR incorporation were used to detect proliferation of VSMCs. Results Doxazosin inhibited the proliferation of VSMCs. The proliferation of NIH3T3 cells,which do not express α 1 receptors, also were inhibited. Pretreatment of cells with phenoxybenzamine, an irreversible α 1 receptor antagonist, did not chang the effect of Doxazosin. Conclusions These results suggest that doxazosin inhibited the proliferation of VSMCs through a mechanism unrelated to α 1 receptor.