摘要
用亨盖特 (Hungate)厌氧技术 ,以甲醇为唯一碳源 ,获得了甲烷八叠球菌的富集培养物。采用直接计数法和MPN(mostprobablenumber)法对甲烷八叠球菌的数量进行测定。选择不同包埋剂对甲烷八叠球菌进行固定化 ,并对其成球效果及产甲烷特性进行了研究。结果表明 ,以AM 4为包埋剂对甲烷八叠球菌进行固定化可得到较好的效果。
Enrichment of Methanosarcina is obtained by using Hungate anaerobic technique and methanol as the sole carbon source. The enumeration of Methanosarcina is conducted using direct enumeration and MPN methods. Immobilized Methanosarcina is accomplished by selecting different entrapment media and the characteristics of immobilized beads and producing methane are demonstrated. The results indicate that AM4 is the best entrapment media for Methanosarcina immobilization.
出处
《太阳能学报》
EI
CAS
CSCD
北大核心
2002年第3期353-356,共4页
Acta Energiae Solaris Sinica
基金
国家自然科学基金 ( 5 9976 0 2 4)
北京市自然科学基金 ( 5 992 0 0 4)资助项目
关键词
甲烷八叠球菌富集培养物
固定化介质
产甲烷特征
enrichment of Methanosarcina
entrapment media
characteristics of producing methane
