微区光谱技术在细胞生物学中的应用

Application of Microscopic Spectrum Techniquefor Cell Biology

建立了能精确测量基于单细胞水平的细胞透过率的微区透射反射光谱仪,应用此装置测量了衣藻细胞和白血病人未加药处理以及药物处理后４８小时的白细胞的透过率曲线．测量结果表明:１)衣藻细胞的吸收峰值与叶绿体的吸收峰值相一致,说明衣藻细胞中吸收光能的细胞器主要是叶绿体;２)两种白细胞的透过率存在明显的差异,说明药物使癌细胞发生了改变．这对于研究怎样有效提高光合作用效率以及临床医学研究中细胞鉴别和药物筛选具有重要的指导意义．

A set of microscopic transmission and reflection spectrum system was set up, which can be use to measure the transmittance of individual cell precisely. With this system, we measured the transmittance of alga cell and leukemic leukocytes with and without medication. The results show that: 1) the absorbing peak of alga cell is consistent with that of chloroplast, from which it can be seen that chloroplast is the major organelle for absorbing energy of light; 2) there is great difference between two kinds of leukocytes in their transmittance, which suggests that the cell was changed with medication. This is instructive on improvement of photosynthesis efficiency, cell identification and drug screening.