TNF-а和IL-10基因多态性预测肾移植效果的临床研究

TNF-α and IL-10 gene polymorphisms in prediction of kidney transplantation outcome

目的 :探讨 TNF-α和 IL - 10基因多态性预测移植肾急性排斥反应的临床意义。 方法 :用序列特异引物聚合酶链反应 (PCR- SSP)方法 ,对 12 6例肾移植患者进行 TNF-α和 IL - 10基因型检测。 结果 :肾移植后急性排斥反应发生率 :TNF-α为高分泌基因型的患者显著高于低分泌基因型者 (P<0 .0 1) ;IL - 10为高分泌基因型的患者显著高于低分泌基因型者 (P<0 .0 5 ) ;TNF-α与 IL - 10均为高分泌基因型的患者比所有其他基因型组合者高 (P<0 .0 1) ,TNF-α与 IL - 10均为低分泌基因型患者比所有其他基因型组合者低 (P<0 .0 5 )。 结论 :常规检测 TNF-α和 IL - 10基因多态性对于预测移植效果具有重要意义。

Objective:To study the significance of TNF α and IL 10 gene polymorphisms for predicting the outcome of kidney transplantation.Methods:TNF α and IL 10 genotypes of 126 consecutive allograft recipients were detected by PCR SSP method. Results:A greater proportion of recipients with TNF α high producer genotype had acute rejection compared with recipients with low producer genotype( P <0.01). Similarly, a greater proportion of recipients with IL 10 high producer genotype had acute rejection compared with recipients with low producer genotype( P <0.05). More recipients with the TNF α high/IL 10 high producer genotype had acute rejection as compared with all the other genotypes( P <0.01). At the same time, fewer patients with the TNF α low /IL 10 low producer genotype had acute rejection as compared with all the other genotypes( P <0.05). Conclusion:Routine screening of these genotypes may play a role in identifying patients with risk of acute rejection.