雾化吸入γ-干扰素增强肺部细胞免疫功能的研究

Effect of IFN-γ inhalation on the cellular immunity of the lungs

目的 :研究雾化吸入γ -干扰素 (IFN -γ)对免疫低下宿主肺部抗感染能力的增强作用。方法 :对气管内注射白色念珠菌和白色念珠菌并雾化吸入IFN -γ(1、3、7d)的免疫低下大鼠 ,检测其血液和肺局部细胞免疫指标 ,并于第 7d检测左肺白色念珠菌培养计数。结果 :雾化吸入γ -干扰素组肺白色念珠菌计数显著少于免疫低下组 ,AM吞噬及杀菌百分率、Ia抗原表达的阳性率显著高于免疫低下组 ,AM培养上清TNF -α活性和IL - 1β、IL - 6水平显著高于免疫低下组 ,BALF中IFN -γ、TNF -α活性高于免疫低下组 (第 7dTNF -α除外 ) ,灌菌后第 7天肺组织IFN -γ和IL - 1β表达高于免疫低下组 ,TNF -α表达低于免疫低下组 ,IL - 6表达两组间无显著差异。雾化吸入IFN-γ组血清IFN -γ活性和IL - 1β、IL - 6水平 (除第 3dIL - 1β外 ) ,与免疫低下组无显著差异 ,且感染后第 7d血淋巴细胞杀伤功能与免疫低下组无显著差异。结论 :雾化吸入γ -干扰素可明显增强免疫低下大鼠肺部细胞免疫和抗感染能力 ,但对全身细胞免疫状态无明显影响。

AIM: To study the effect of IFN-γ inhalation on the anti-infection ability of the lungs in the immunocompromised host. METHODS: The immunological factors in the immunocompromised rats and the immunocompromised rats administrated IFN-γ via aerosol were investigated after 1, 3, 7 days when they were injected Candida albicans via tracheal The Canidda albicans count of the left lung was also determined after 7 days when injecting pathogen. RESULTS: The Canidda albicans count of the left lung in IFN-γ group was significantly less than that of control group. The phagocyting and bactericidal percentages, Ia antigen expression percentages, the levels of TNF-α, IL-1β and IL-6 in the culture supernatant of the AM, the activity of IFN-γ and TNF-α in BALF (except the TNF-α on 7 th day) in IFN-γ group were markedly higher than those in control group. The expression of IFN-γ and IL-1β pulmonary tissues in IFN-γ group was higher than that in control group. The expression of TNF-α in IFN-γ group was less than that in control group. The expression of IL-6 was no changes between two groups. The levels of IFN-γ, IL-1β and IL-6 in the blood (except IL-1β on 3 rd day), and the killing ability of the lymphocytes in blood had no difference between two groups. CONCLUSION: Administration of IFN-γ via aerosol obviously enhanced the anti-infection ability of the lungs in the immunocompromised host, but has no influence on the whole body cellular immunity.