补肾益精方对卵巢切除后骨质疏松大鼠皮质骨截面几何结构、骨密度和生物力学性能的影响

EFFECTS OF BSYJF, A TRADITIONAL CHINESE PRESCRIPTION, ON MACROSCOPIC GEOMETRY STRUCTURE OF SECTION, BONE DENSITY AND BIOMECHANICAL PROPERTIES OF CORTICAL BONE OF FEMUR IN OSTEOPOROTIC RAT MODEL INDUCED BY OVARIECTOMY

目的 评价补肾益精方提高绝经后骨质疏松症大鼠模型皮质骨质量的有效性 ;探讨其改善皮质骨力学性能的作用机制。方法 Wistar雌性大鼠 4 0只随机分为A组 (正常对照组 ,双侧卵巢假切除术 )、B组 (模型对照组 ,双侧卵巢切除术 )、C组 (补肾益精方组 ,双侧卵巢切除术 +补肾益精方 ) ,D组 (倍美力组 ,双侧卵巢切除术 +倍美力 )。给药组的给药时点是双侧卵巢切除术后 90天 ,处死时点是卵巢切除术 /假切除术后 1 80天。处死后 ,测定各组大鼠左股骨中段的截面宏观几何结构参数、骨矿密度与右股骨三点弯曲的结构力学参数、材料力学参数。结果 截面宏观几何结构参数显示 ,B组与A组比较 ,骨髓腔显著扩大 (P <0 0 5 ) ,骨皮质显著变薄 (P <0 0 5 ) ;C组、D组与B组比较 ,骨髓腔都没有明显缩小 ,但骨皮质均明显变厚 ( P <0 0 5 )。B组的骨密度与A组比较 ,没有明显下降 ;C组也无明显上升。B组的结构力学和材料力学参数与A组比较 ,均显著下降 (P <0 0 5 ) ;C组、D组与B组比较 ,C组的结构硬度与弹性模量显著上升 (P <0 0 5 ) ,D组上升不显著 ,两组其余的力学参数都显著上升 (P <0 0 5 )。结论 补肾益精方可以提高双侧卵巢切除后骨质疏松症大鼠皮质骨的骨质量。其作用机制是修饰皮质骨截面的宏观几何结构 ;而与?

Objective To evaluate the efficacy of BSYJF on raising the quality of cortical bone of femur in osteoporotic rat model induced by ovariectomy and its mechanism on improving biomechanical properties of cortical bone. Method Forty female Wistar rats were divided randomizedly into four groups: Sham control group(GA), Ovx control group(GB), BSYJF group(Ovx+BSYJF,GC) and BML Group(Ovx+BML, GD). The time point of administering drug(BSYJF or BML) to the rats was 90 days after ovariectomy. The time point of sacrificing was 180 days later. After sacrificing, macroscopic geometry structure, bone mineral density and bionechanical properties of three-point bending (structural mechanical property and material mechanical property) of femur were determined. Results In GB, the dirmeter of medullary canal was enlarged significantly (P<0.05) and the thickness of cortical bone decreased significantly (P<0.05), respectively vs GA; in GC and GD, there was no significant change of the diameter but the thickness of cortical bone all was enlarged olveously (P<0.05), respectively vs GB. The bone mineral density in GB were not decreased significantly as compared with GA also in GC compared with GB. In GB the parameters of structural mechanical property(maximum load. stiffness and energy) and the parameters of material mechanical property(maximum stress and elastic modulus) were decreased significantly (P<0.05), respectively compared with GA. Stiffness and elastic modulus in GC were increased significantly (P<0.05) but not in GD (P<0.05) and other parameters of mechanical properties in the two groups (GC and GD) were all increased sinificantly (P<0.05), respectively compared with GB. Conclusion BSYJF can raise the quality of cortical bone of femur in the rat model induced by ovariectomy. In improving biomechanical properties of cortical bone, BSYJF has the same partial mechanism, which can modify the macroscopic geometry structure of cortical bone as BML. But BSYJF has another different mechanism, which can up-regulate stiffness and elastic modulus of cortical bone without increasing bone mineral density.