GC-MS法建立广藿香挥发油指纹特征图谱研究

Study on characteristic fingerprint of volatile oil of Pogostemon cablin (Blanco) Benth by GC-MS

目的 :建立广藿香挥发油指纹特征图谱 ,为广藿香的内在质量评价和鉴定提供特征数据。方法 :采用GC MS法对不同产地广藿香 (石牌、高要、海南藿香 ) ,组织培养广藿香 ,以及超临界提取广藿香、广藿香对照品共 2 1个样品进行主成分分析。结果 :2 1个广藿香样品均含有 β 广藿香烯、β 榄香烯、顺式 石竹烯、反式 石竹烯、刺蕊草烯、α 愈创木烯、α 广藿香烯、δ 愈创木烯、未鉴定A、广藿香醇、广藿香酮等主要成分 ,以上 11种主成分相对含量约占挥发油总量的 80 %左右。石牌藿香与高要、海南藿香比 ,11个成分的相对含量 (除顺式 石竹烯、反式 石竹烯外 ) ,均有显著性差异 ;高要藿香与海南藿香比无显著性差异。结论 :11个主要成分的出峰先后顺序及相对含量极具特征性 ,形成了广藿香特有的指纹图谱 (地貌 ) 。

Objective: To establish the characteristic fingerprint of volatile oil of Pogostemon cablin (Blanco) Benth.Methods: The main components of 21 samples were determined by GC MS. Results: β Patchoulene, β Elemene, cis Caryophyllene, trans Caryophyllene, seychellene, α Guaiene, α Patchoulene, δ Guaiene, unidentification component A, patchoulic alcohol, pogostone were determined in all samples, and the relative content of 11 components add up to 80 percent. Compared with the samples produceds in Gaoyao and Hainan, Shipai samples were significantly differences.Conclusion: The retention time and relative content of 11 components bulidup the characteristic fingerprint of volatile oil of Pogostemon cablin (Blanco) Benth, it can be used for quality control and identification to Pogostemon cablin (Blanco) Benth.