同时检测多种心血管疾病相关基因多态性的方法

A Multilocus Genotyping Assay for Candidate Genotypes of Cardiovascular Disease

应用Roche分子系统提供的试剂、材料和实验操作步骤 ,介绍其建立的一种同时检测多种心血管疾病相关基因的多个位点基因型分析方法 ,并且比较所检测的不同中国地区 (北京和香港 )的中国人群之间和中国人与法国人群之间的各等位基因频率的差异。结果发现 ,北京人群和香港人群在所有检测的等位基因频率方面没有显著差别 ,但是中国人和法国人在一些等位基因频率分布方面存在显著差异。总之 ,Roche分子系统建立的是一种快速而有效的分析多基因多位点基因型的方法 ,有益于大规模的人群调查心血管疾病的遗传危险因素。

Aim To introduce a multilocus genotyping assay for candidate genotypes of cardiovascular disease (CVD) established by Roche Molecular Systems (RMS). Methods By applying the regents, materials and the protocol provided by RMS, genotyping was done for the Chinese from different regions (Beijing and Hong Kong) and a French population. Results By comparing the allelic frequencies between the Chinese from different regions and between the Chinese and the French populations, no significant difference was found for each allelic frequency between the two Chinese groups, but some allelic frequencies significantly differed between the Chinese and the French populations. Conclusions This multilocus genotyping assay established by RMS is a rapid, convenient and efficient method in determining the genotypes possibly related to the development of CVD in a large scale population study for the genetic risk of CVD.