厚朴的任意引物PCR指纹图谱分析

Fingerprint of arbitrarily primed polymerase chain reaction (AP-PCR) for Magnolia officinalis

目的 鉴别中药材厚朴、凹叶厚朴及其常见的伪品和混淆品。方法 采用任意引物 PCR (arbitrarilyprim ed PCR,AP- PCR)技术扩增植物基因组 DNA样品。结果 AP- PCR技术获得清晰可靠的 DNA指纹图谱 ,根据琼脂糖凝胶上显示的 DNA带型差异可迅捷地区分厚朴、凹叶厚朴及其伪品、混淆品。结论 为应用 AP- PCR技术在分子水平上鉴别中药材厚朴 ,保证引种的准确性奠定了基础。

Object To identify Magnolia officinalis Rehd et Wils and M biloba Rehd et Wils as well as their counterfeits and easily confusable substitutes Methods Total genomic DNA samples of ten plant species were amplified by arbitrarily primed PCR (AP PCR) Results Ten samples were distinguished through their amplified DNA banding patterns on the agarose gels after eletrophoresis, so as to differentiate the counterfeits and easily confusable substitutes of M officinalis and M biloba. Conclusion AP PCR is useful for identifying M officinalis at molecular level, as well as valuable to guarantee introducing original plant correctly