摘要
本研究针对婴儿双歧杆菌的营养需要 ,采用正交实验优化基础培养基及增殖因子的用量。得出最佳基础培养基配比是 :大豆蛋白胨 1.2 % ,胰蛋白胨 0 .6 % ,酪蛋白水解物 0 .6 % ,牛肉浸膏 0 .6 % ,葡萄糖 0 .5 % ,低聚糖 1.0 % ,乳糖 0 .5 % ,酵母浸出粉 0 .5 %。最佳增殖因子添加量是 :胡萝卜汁 15 % ,西红柿汁 10 % ,肝浸液 5 % ,胱氨酸 0 .0 5 %。最后使用优化出的增菌培养基配比测定生长曲线 ,同时测定pH和滴定酸度的变化 ,确定增菌培养终止时间为 10h,通过平板菌落计数 ,此时菌数可达 8.78×10
According to the nutritional needs of B. infantis, based culture and stimulated factors were optimized by quadratic design. The optimum based culture was as following:soya peptone was 1.2%,tryptone was 0.6%,casein enzymatic hydrolysate was 0.6%,beef extract was 0.6%,glucose was 0.5%,oligofructose was 0.1%,lactose,yeast extract was 0.6%,The optimum stimulated factors were as following:carrot juice was 15%,tomato juice was 10%,liver infusion was 5%,cystine was 0.05%,Growth curve with optimized stimulated culture were determined, with the change of pH and titratable acidity. According to the growth curve,the terminal time was at 10 hours.B.infantis got to 8.78×10 9cfu/mL in this culture for 10 hours at 37℃ under anaerobic condition.
基金
国家自然科学基金资助项目 (39870 573)
黑龙江省教育厅科技重大项目 (1 0 81 1 70 0 3)