三尖杉酯碱与牛血清白蛋白相互作用的研究

A Study on binding of harringtonine to bovine serum albumin

本文于体外进行了天然抗肿瘤药物三尖杉酯碱与牛血清白蛋白相互作用的研究,采用平衡透析法(pH=7.40±0.01,μ=0.2)在恒温25±1℃,振荡频率为110～120次/min,振荡16～18h的条件下,测定了其结合参数。实验结果表明,当三尖杉酯碱浓度为8.5×10^(-4)～1.55×10^(-3)mol/L,牛血清白蛋白浓度固定为4.5×10^(-4)mol/L时,其结合常数为3.51×10~2,结合部位数为0.93即三尖杉酯碱对牛血清白蛋白只有一个结合部位群。经荧光光谱法检测,证明该结合部位群在牛血清白蛋白分子的U部位。

Binding of harringtonine (HT) to bovine serum albumin (BSA) was studied in vitro at constant temperature 25±1℃, frequency of vibration 110-120 times per min using equilibrium dialysis method (pH=7.40±0.01, μ=0.2). The result showed that the binding constant and the number of binding sites were 3.51×10~2 and 0.93 respectively, when the concentrations of HT were applied over the range of 8.5×10^(-4) mol/L and 1.55×10^(-3) mol/L and BSA concentration was fixed at 4.5×10^(-4) mol/L. HT has only one binding site and the binding site was U site on BSA by fluorescence spectra method.