淡紫拟青霉最佳液体培养基和发酵条件研究

OPTIMUM LIQUID CULTURE MEDIUM AND FERMENTATION CONDITIONS FOR PAECILOMYCES LILACINUS

采用正交实验对淡紫拟青霉IPC菌株的发酵培养基进行了研究 ,得到了适合其发酵培养的复合培养基配方 ,另外试验还证实适当增加通气量、发酵初始酸碱度控制在pH 5～ 6、发酵温度在 2

The culture media for the IPC strain of Paecilomyces lilacinus were studied using the orthogonal design. Best fermentation was achieved on the medium of maize powder 40 g/L + soybean powder 30 g/L + FeSO 4·7H 2O 0.005 g/L + MnSO 4 0.008 g/L + H 3BO 3 0.002 g/L + ZnSO 4 0.005 g/L + CuSO 4 0.004 g/L + Na 2MoO 4 0.004 g/L + CoCl 2 0.002 g/L + cyclohexanhexol 0.04 g/L. The results of the experiment showed that enhanced aeration, an initial pH of 5～6 and a temperature of 29℃ were favorable for the deep fermentation for this strain of P. lilacinus.