土壤食细菌线虫的分离和富集培养方法

Isolation and enrichment culturing of a soil bacterial-feeding nematode

从小麦根际土中分离得到一种能取食B1和B2两种细菌的土壤食细菌线虫 ,并在马铃薯蔗糖培养基上富化培养成功。经鉴定该线虫为原小杆线虫属 (Protorhabditissp .) ,B1为假单胞菌属 (Pseudomonassp .) ,B2为醋杆菌属(Acetobactersp .)。用牛肉膏蛋白胨细菌培养基培养B1和B2两种细菌时 ,细菌无多糖产生 ,线虫不能取食细菌而生长繁殖 ;当用马铃薯蔗糖真菌培养基培养B1和B2两种细菌时 ,细菌分泌丰富的多糖 ,线虫生长繁殖较好。

A soil bacterivorous nematode feeding on bacteria B1 and B2 was isolated from rhizosphere soil of wheat and cultured on potato sucrose agar medium(PSA).The nematode was identified as Protorhabditis sp.;B1 and B2 were identified as Pseudomonas sp.and Acetobacter sp.,respectively.B1 and B2 didn't secrete polysaccharose on beef polypeptone agar medium(BPA) and the nematode didn't grow on BPA medium with B1 and B2,while B1 and B2 secreted polysaccharose on PSA medium.The nematode grew very well on PSA medium with B1 and B2.Polysaccharose constitutes an important external environment for nematode growth.