迟缓爱德华菌外膜蛋白抗原性分析

Characterization of antigenicity of the outer membrane protein from Edwardsiella tarda

目的 :分析迟缓爱德华菌 (Et)外膜蛋白 (OMP)的抗原性。方法 :用Western blotting分析 2 7株Et与EtATCC15 94 7OMP抗血清的转印情况 ,用ELISA、杀菌力试验和凝集反应检测OMP诱导的抗体滴度。结果 :2 1株致病株中有 2 0株能与EtATCC15 94 7株OMP抗血清转印出清晰的条带 ,分别为 33k、35k、38k、4 5k ,而非致病株和另一株致病株Et12 2的OMP则没有反应带 ;ELISA、杀菌力试验和凝集反应表明 ,ATCC15 94 7和JEL4株的OMP均能诱导高滴度的杀菌抗体和抗OMP抗体 ;不同程度稀释的抗ATCC15 94 7OMP血清及抗 35k、4 5k血清还能对小鼠提供一定的保护力。结论 :EtOMP33k、35k、38k、4 5k可能为保护性抗原 ,OMP可作为疫苗的候选成分。

Objective:To study the antigenicity of OMP extracted from Edwardsiella tarda.Methods:ELISA, Bactericidal test, Agglutinating test and Western blotting were used for testing the antigenic titers and immunogenicity of OMP.Results:In immunoblotting, by using ATCC15947 OMP antibody, the non pathgenic strains were negative, while all pathogenic strains except Et 122 gave positive results and had OMP bands of 33k, 35k, 38k, and 45k. OMPs of both ATCC 15947 and JEL4 could induce high antibody titers. Further more, the antibodies evoked by OMPs of ATCC 15947 of 33k or 35k could also protected mice to some degree when diluted.Conclusion:The 33k, 35k, 38k, and 45k of OMPs may be protective antigens, and the OMPs of Et could be a candidative component for vaccine.