HER-2/neu在卵巢上皮性肿瘤中的表达及意义

Significance of HER-2/neu expression in ovarian epithelial tumours

目的 研究HER - 2 /neu(c -erbB - 2 )在卵巢上皮性肿瘤中的表达及意义。方法 收集 10 6例卵巢上皮性肿瘤 (恶性 5 4便 ,交界性 33例 ,良性 19例 )及临床资料。按国际妇产科联合会(FIGO)标准进行分期 ,卵巢中Ⅰ、Ⅱ期共 19例 (19/ 5 4 ,35 .2 % ) ,Ⅲ、Ⅳ期 35例 (35 / 5 4 ,6 4 .8% ) ,交界性肿均为Ⅰ期。采用标记链霉素卵白素生物素 (LSAB)法用HER - 2 /neu多克隆抗体 (DAKO ,A0 4 85 )行免疫组织化学染色。结果 该蛋白表达的阳性率在良性肿瘤为 4 7.4 % (9/ 19) ,过表达 (3+)为 0 ;交界性肿瘤为 84 .8% (2 8/ 33) ,过表达率为 9.1% (3/ 33) ;恶性肿瘤为 85 .2 % (46 / 5 4 ) ,过表达率为 2 5 .9%(14 / 5 4 )。良性与交界性以及良性与恶性之间的HER - 2 /neu蛋白表达率的差异有显著性 (P〈 0 .0 2 ,P〈0 .0 1) ;该蛋白的过表达率在有转移和无转移的病例之间差异有显著性意义 (P〈0 .0 0 1)结论 HER- 2

Objective To investigate expression of HER-2/neu(c-erbB-2) and its significance in ovarian epithelial tumors.Methods 106 specimens and clinical history of ovarian epithelial tumors were collected including 54 cases of malignant,33 cases of borderline and 19 benign cases.There were 19 cases of stages Ⅰ and Ⅱ and 35 cases of stages Ⅲ and Ⅳ in ovarian carcinoma and all borderline malignant cases were stage Ⅰ according to FIGO's standard.Immunohistochemical staining for Her-2/neu was performed by LSAB method.Results The positives rates of Her-2/neu of benign,borderline and malignant tumors were 47.4%(9/19),84.8%(28/33,),and 85.2%(46/54),respectively.Both borderline and malignant cases were found to have higher expression of Her-2/neu than those of benign cases (P < 0.02 and P<0.01) respectively.Overexpression of Her-2/neu showed in 14/54(25.9%) of malignant cases,3/33(9.1%) of borderline,and none of benign cases.Overexpression of Her-2/neu in malignant tumors cases with metastasis was higher than those without metastasis (P<0.001) .Conclusions Overexpression of Her-2/neu is associated with biological aggressiveness of ovarian cancer.