地塞米松对成纤维细胞增殖及表达细胞间粘附分子-1的影响

Effects of dexamethasone on proliferation and expression of intercellular adhension molecules by human oral fibroblasts

目的 :探讨地塞米松 (DEX)治疗口腔粘膜下纤维性变 (OSF)等口腔粘膜疾病的机理。方法 :分别从正常 (NM)及OSF患者的口腔粘膜中培养出成纤维细胞 (FB) ,向培养基中加入不同浓度的DEX培养 4 8h后 ,用MTT法检测FB的增殖水平 ,并用细胞酶联免疫方法检测其细胞间粘附分子 1(ICAM 1)的表达水平。结果 :DEX在 10～ 15 0 μg ml的范围内以浓度 效应依赖关系抑制FB增殖 ;表示ICAM 1水平高低的OD值在OSF FB为 0 386± 0 0 99,高于NM FB的OD值 0 32 4± 0 0 30(P <0 0 5 ) ;DEX可下调FB的ICAM 1表达水平。结论 :DEX可能是通过抑制FB增殖和下调ICAM 1的表达而达到治疗OSF等口腔疾病的效果。

Objective:Dexamethasone (DEX) has been proved to be an effective therapy for a variety of oral mucosal disorders, especially for oral submucous fibrosis(OSF) .The mechanisms behind its therapeutic effects are not known but have largely been ascribed to its anti proliferation and immunosuppressive effects. The recruitment, extravagation and retention of leucocytes which may play an important role in the development of oral mucosal disorders depends in part on the interaction of LFA 1 with its ligand intercellular adhension molecules(ICAM 1).The study aimed to investigate the effects of DEX on proliferation and expression of ICAM 1 by human oral fibroblasts (FB).Methods:The fibroblasts were obtained from normal buccal mucosa (NM FB) and OSF buccal mucosa (OSF FB) and cultured in vitro. Then the cell proliferation of fibroblasts incubated with or without DEX in the presence of 10%fetal calf serum for 48 hours at 37℃ in 5%CO 2 and air were monitored by use of thiazolyl blue (MTT) assay and the level of ICAM 1 expressed by fibroblasts were monitored by using cell based ELSA for ICAM 1.Results:OSF FB had an increased proliferation compared to NM FB and DEX inhibited fibroblast proliferation in a concentration dependent manner; OSF FB produced ICAM 1 at high levels and DEX decreased ICAM 1 expression levels on both cell types.Conclusion:DEX can directly inhibit human oral fibroblasts proliferation and reduces the levels of ICAM 1 expressed by oral mucosal FB, and may be useful in the treatment of some oral mucosal disorders.