胆管癌组织中自发性细胞凋亡现象的研究

A Study on Spontaneous Apoptosis in Cholangiocarcinoma

目的:探讨胆管癌组织中自发性细胞凋亡现象的意义。方法:TUNEL染色结合形态观察原位检测凋亡细胞。结果:40例胆管癌标本的中位SAI高达7.89%,高/中分化和无淋巴结转移胆管癌的SAI分别显著高于低分化和淋巴结转移者(P<0.001)。结论:胆管癌仍保留有相对活跃的细胞凋亡机制,SAI可作为判断胆管癌恶性程度的有益指标。

Objective:To investigate the clinicopathological significance of spontaneous apop-tosis in cholangiocarcinoma.Methods:TUNEL staining technique was conducted to determine apoptotic cells in situ in cholangiocarcinoma tissues.Results:The median of spontaneous apop-totic index(SAI)was up to7.89%in40cases of cholangiocarcinoma,and the SAIs in cases well differentiated and without lymph node metastasis were significantly higher than those in cases poorly differentiated and with lymph node metastasis respectively(P<0.001).Con clusion:The apoptosis mechanism is well preserved in cholangiocarcinoma,and SAI is potentially useful to pre-dict the malignant grade of cholangiocarcinoma.