急性白血病细胞bcl-2和p53基因表达的研究

The Studies of the expression of bcl-2 and p53 genes in acute leukemia.

目的 研究bcl- 2和p5 3基因在急性白血病 (AL)细胞中的表达及其与AL的分型、临床特征、疗效及预后的关系。方法 采用免疫组化方法检测 5 4例初治AL骨髓细胞bcl- 2和p5 3蛋白表达情况 ,分析其与FAB分型、临床特征、疗效及预后的关系。结果 AL骨髓细胞中bcl- 2及p5 3基因的表达显著高于正常对照组(P <0 0 5 ) ,而在急性髓系白血病 (AML)与急性淋巴细胞白血病 (ALL)中的表达无显著差异 ;在AML亚型中 ,bcl- 2在M4 及M5中的表达高于M1,M2 和M3 (P <0 0 5 ) ;而p5 3的表达在各亚型中无显著性差异 (P>0 0 5 )。bcl- 2的表达与初诊时的白细胞数呈正相关 (R =0 4 4,P <0 0 5 ) ,与疗效呈负相关 (R =- 0 4 4,P <0 0 5 )。结论 bcl- 2和p5 3基因的紊乱可能达AL的发病中起作用 ,还与白血病的某些临床特征、疗效及预后密切相关。

Objective To study the expression of bcl-2 and p53 genes in acute leukemia (AL) and their significance in the FAB classification.Methods By using immunohistochemical staining method analysis,the expressions of bcl-2 and p53 genes in 54 cases of untreated AL were detected,and the correlation of these genes with FAB classification,some clinical features,treatment outcome and prognostic factors of AL were also studied.Results Overexpressions of bcl-2 and p53 genes were found in AL(P<0.05).According to FAB classification,the expression of bcl-2 and p53 in acute myeloid leukemia(AML)and acute lymphoid leukemia (ALL)had no significance.The expression of bcl-2 was higher in type M 4 and M 5 than M 1,M 2 and M 3(P<0.05).The expression of p53 in various types had no significance(P>0.05).Conclusion Abnormal expressions of bcl-2,and p53 genes in AL indicate that they play a pivotal role in pathogenesis and progression of AL. These apoptosis-regulating genes are still related to some clinical features, treatment outcome and prognostic factors of leukemia.