摘要
以PM2 6 44为受菌体 ,通过基因功能互补的方法 ,将从野生青枯菌基因文库中筛选得到的克隆进一步克隆得到克隆pLTK15。胞外酶活性测定结果表明 ,pLTK15与突变株PM2 6 44接合产生的接合子能完全恢复突变株PM2 6 44聚半乳糖醛酸酶的活性 ,接合子上清液SDS PAGE电泳结果表明 ,接合子只能互补PM2 6 44所缺失的 6 2 .5kDa的一种胞外蛋白 ,经致病力测定 ,pLTK15能使突变株PM2 6 44的致病力得到部分恢复。结果表明 ,聚半乳糖醛酸酶和 6 2 .
The clone(pLTK15) was cloned from the clone which were screened out from genomic library of wild type stain PO41 by gene function complementation test. pLTK15 related to extracellular protein of Ralstonia solancearum. The complementary conjugants of PM2644 with pLTK15 restored all of the activity of polygalacturonase.The result of SDS PAGE showed that it restored only one kind of the lost extracellular proteins(62.5 kDa). The virulence of the complementary conjugants of PM2644 with it can be partially restored .The results showed that polygalacturonase and the 62.5 kDa extracellular protein play a role in the process of Ralastonia solanacearum infect plants.
出处
《浙江农业学报》
CSCD
2002年第3期131-134,共4页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金重点资助项目
