二氮杂芴-9-酮的高效液相色谱分析

Determination of 9H-Cyclopenta[1,2-b:4,3-b′]dipyridin-9-one by HPLC

采用KromasilC18柱为分离柱 ,甲醇 -水 (体积比 6 0∶4 0 )为流动相 ,检测波长为 2 5 4nm ,苯乙酮做内标 ,对二氮杂芴 9 酮的含量进行了液相色谱定量检测。在 8～ 180 μg/mL峰面积 (y ,Ai/As)对标准溶液的质量浓度 (x ,μg/mL)线性关系良好 (r =0 99996 ) ,该法快速。

A high performance liquid chromatographic method is reported for the quantitative determination of 9H cyclopenta[1,2 b:4,3 b′]dipyridin 9 one(Ⅰ)using Kromasil C 18 column as a separation column and CH 3OH-H 2O(60∶40 by volume) system as a mobile phase.Ⅰ was quantitatively determined by internal standard (acetophenone )method at the wavelength of 254 nm.The calibration curve was in good linearity ( r =0 99996) over the mass concentration of 8-180 μg/mL for Ⅰ.This method is specific, rapid and accurate.