摘要
采用KromasilC18柱为分离柱 ,甲醇 -水 (体积比 6 0∶4 0 )为流动相 ,检测波长为 2 5 4nm ,苯乙酮做内标 ,对二氮杂芴 9 酮的含量进行了液相色谱定量检测。在 8~ 180 μg/mL峰面积 (y ,Ai/As)对标准溶液的质量浓度 (x ,μg/mL)线性关系良好 (r =0 99996 ) ,该法快速。
A high performance liquid chromatographic method is reported for the quantitative determination of 9H cyclopenta[1,2 b:4,3 b′]dipyridin 9 one(Ⅰ)using Kromasil C 18 column as a separation column and CH 3OH-H 2O(60∶40 by volume) system as a mobile phase.Ⅰ was quantitatively determined by internal standard (acetophenone )method at the wavelength of 254 nm.The calibration curve was in good linearity ( r =0 99996) over the mass concentration of 8-180 μg/mL for Ⅰ.This method is specific, rapid and accurate.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2002年第5期302-303,共2页
Fine Chemicals
关键词
二氮杂芴-9-酮
苯乙酮
HPLC
内标法
H cyclopenta[1,2 b:4,3 b′]dipyridin 9 one
acetophenone
HPLC
internal standard method