摘要
我们首次在国内提纯了光感受器间维生素 A类结合蛋白(interphotoreceptor retinoid-binding protein,IRBP),将其免疫 Lewis 大鼠后动态测定了鼠血清抗IRBP 抗体和抗视网膜 S 抗原抗体.发现抗 IRBP 抗体于免疫后第7天出现,以后逐渐上升,于第26天达高峰,未测出抗 S 抗原抗体.根据特异性抗体与实验性自身免疫性葡萄膜视网膜炎(experimental autoimmune uveoretinitis,EAU)之间的关系,讨论了特异性体液免疫反应在 EAU发生中的作用.
The serum antibodies specific to either inter-photoreceptor retinoid-binding protein (IRBP) or retinal S- antigen were longitudinally determined in Lewis rats immu-nized with 50×10^(-6)g of IRBP emulsified with complet Fre-und's adjuvant.To our knowledge,it is the first time to pu-rify IRBP and to address the experiment with IRBP in China.It was shown that the antibody specific to IRBP appeared onthe 7th day,followed by a gradual increase,and reached itspeak on the 26th day after immunization.No antibody spe-cific to S-antigen was found.The relaton between anti-IRBPantibody and experimental autoimmune uveoretinitis(EAU)which occurred in the increasing phase of the antibody sug-gests that the humoral immunity may be implicated in thepathogenseis of EAU.
出处
《眼科学报》
1991年第2期54-57,共4页
Eye Science
