“云克”对白细胞介素(IL)-1β刺激的人眼球后成纤维细胞的影响

EFFECTS OF 99Tc-MDP ON THE CULTURED RETROOCULAR FIBROBLAST TO STIMULATED BY IL-1β

目的 :探讨云克治疗Graves眼病的作用机制。方法 :应用细胞培养技术 ,测定加入白细胞介素 (IL - 1β)及“云克”后细胞间粘附分子 (ICAM ) - 1的表达和DNA合成情况。结果 :白细胞介素 (IL) - 1β(10 0U/ml)能强烈刺激眼球后成纤维细胞表面ICAM - 1表达 ,而云克明显地抑制这种刺激作用 ;同样 ,云克能抑制IL - 1β刺激的DNA合成。结论 :云克治疗GO的作用途径部分是通过抑制白细胞介素 - 1β的作用 ,使ICAM - 1表达及DNA合成减少。

Objective:To investigate the mechanism by which 99 Tc-MDP treats Graves' opthalmopathy (GO).Methods:ICAM-1 expression and DNA synthesis in cultured retroocular fibroblasts incubated with IL-1β and 99 Tc-MDP were measured.Results:IL-1β (100U/ml) could significantly stimulat the ICAM-1 expression in retroocular fibroblasts. 99 Tc-MDP markedly inhibited these stimulations. 99 Tc-MDP also could inhibit the DNA synthesis stimulated by IL-1β.Conclusion: 99 Tc-MDP treats GO partially by inhibiting the effect of IL-1β and decreasing the expression of ICAM-1.