肿瘤坏死因子联合足叶乙甙对肺癌细胞株A549的毒性作用及临床意义

The Significance of TNFα Combined with VP16 on Growth of Lung Cancer Cell Line

目的 :研究肿瘤坏死因子 (TNFα)和足叶乙甙 (VP16 )联合抗肿瘤的作用及其机制。方法 :用MTT法检测不同浓度的TNFα、VP16及TNFα/VP16对人肺癌细胞系A5 49的细胞毒作用及流式细胞仪观察TNFα、VP16及TNFα联合VP16分别对A5 49的细胞周期的影响。结果 :TNFα、VP16对A5 49细胞的生长抑制作用有时相及剂量依赖关系 ,而TNFα联合VP16对A5 49抑制作用较TNFα、VP16单个作用显著 (P <0 .0 1) ,对细胞周期用药后G2 期明显下降 ( 13.2 %下降至 3.0 % ,S期上升 ( 2 7.3%上升至42 .5 % ) (P <0 .0 5 )。结论 :VP16联合TNFα可增加对肺癌细胞A5 49的抑制率 ,对A5 49细胞的作用是通过抑制DNA合成及有丝分裂使G0 +G1+S期不能及时转至G2 、M期。

Objective: To investigate the cytotoxic activities of tumor necrosis factor-α and VP16 on human lung cancer line cells A549. Methods: A wide range of TNFα concentration (from 10～160 ng/ml) and VP16 (10～80 μmol/L) were tested using the MTT assay. The cell cycle of A549 cells was measured by flow cytometric analysis (FCM). Results: Data showed that the suppressive effects of factor-α and VP16 on A549 were dose-dependently and the suppressive effect of TNFα combined with VP16 was better than TNFα or VP16. After 4 hours of exposure of A549 cell to TNFα and VP16 the cycle of cells shifted from G 2 phase to S phase. Conclusions: TNFα with VP16 could inhibit the proliferation of A549 cells. It is suggested that the mechanism of TNFα with VP16 on tumor cell is to interfere with the cycle.