呼吸道合胞病毒引起的下呼吸道感染中血清IL-4、IL-12水平的相关性

The Levels of Serum IL-4 and IL-12 in the Children with Lower Respiratory Tract Infection Caused by Respiratory Syncytial Virus

目的 :探讨IL 4和IL 12在呼吸道合胞病毒 (RSV)引起的下呼吸道感染中所起的作用。方法 :①用碱性磷酸酶抗碱性磷酸酶桥联酶标法 (APAAP)检测出 30例RSV抗原阳性患儿 ;②应用双抗体夹心酶联免疫吸附法 (ELISA)分别检测患儿和正常儿童血清中IL 4、IL 12水平并进行相关分析。结果 :①治疗前RSV下呼吸道感染患儿血清IL 4水平显著升高 ,IL 12水平显著降低 ,与对照组比较有统计学意义 (P <0 .0 0 5 )。②治疗后病情好转患儿的IL 4水平下降 ,IL 12水平升高 ,与正常对照无显著性差别 (P >0 .0 5 )。③血清IL 4、IL 12之间有显著负相关关系 (r =-0 .432 ,P <0 .0 5 )。结论 :RSV下呼吸道感染存在Th1/Th2功能紊乱 ,主要表现Th2细胞功能亢进 ,IL 4、IL 12参与了RSV下呼吸道感染时的免疫病理机制。

Objective: To investigate the role of interleukin-4(IL-4) and interleukin-12(IL-12) in the children with lower respiratory tract infection caused by respiratory syncytial virus (RSV). Methods: ①RSV antigen in the nasopharyngeal cell was detected by APPAAP and the patients with RSV antigen positive were selected. ②Serum levels of IL-4 and IL-12 were determined before and after treatment respectively by enzyme linked immunosorbent assays(ELISA) and analysed by linear correlation in 30 patients, and compared with normal control group. Results: ① the level of IL-4 was significantly higher( P <0.005), while the level of IL-12 was significantly lower( P <0.01) in most patients before treatment than those of the control subjects. Serum level of IL-4 was significantly decreased and the level of IL-12 was significantly increased in patients after treatment. ② There was a negative correlation between IL-4 and IL-12 ( r =-0.432, P <0.05). Conclusions: Th1/Th2 functional imbalance exists in patients with lower respiratory tract Rsv infection, and Il-4, IL-12 are involved in the immunopathological mechanisms of RSV infection.