紫萁快速繁殖技术的研究

Studies on the Rapid Propagate of the Osmunda japonica Thund.

研究了影响紫萁孢子萌发的因素。紫萁孢子萌发的最佳培养基为 1 /8MS培养基 ,孢子接种密度以 3 0 0 0个 /cm2 为宜。孢子萌发后逐渐长成丝状体、片状体和原叶体。孢子萌发 4个月后开始形成孢子体。有利于原叶体生长分化和孢子体生长的培养基为 1 /8MS +6 BA 1mg/L +NAA 0 .5mg/L ,孢子体生根培养基以 1 /8MS+6 BA 0 .5mg/L+NAA 1mg/L为宜。紫萁幼苗的生长与阳光、温度、湿度密切相关。

The factors of influencing spore germination was studied in this paper.The suitable culture medium and spore density for Osmunda japonica Thund.spores was 1/8 MS and 3000/cm 2.After germinating,spores grew into the filiform shape,flaky,prothallium and developed into sporophyte 4 months later.The optimized culture medium for prothallium to disintegrate sporophyte was 1/8 MS+6 BA 1+NAA 0.5 and culture medium of root growing was 1/8 MS+6 BA 0.5+NAA 1.0.The growth of seedlings was closely related to sunlight, temperature and humidity.